Product Instructions
Mycelio’s Method: Product Instructions
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Welcome to the definitive guide for cultivating your own mushrooms! Our detailed, step-by-step instructions are designed to help beginners achieve a successful harvest.
Whether you’ve chosen our convenient All-in-One Mushroom Grow Bag or one of our beginner-friendly Mushroom Grow Kits you’ll find all the guidance you need right here. Simply navigate to the instructions that match your product and start your journey to a fruitful mushroom harvest.
Grain Spawn Bags
Mycelio's Sterile Grain Spawn Bags
Mycelio’s sterile grain spawn bags are designed to give cultivators a clean, reliable start. Each 3lb bag is pressure-sterilized and sealed under laminar flow, equipped with a self-healing injection port and a 0.2-micron filter patch to support consistent colonization.
We offer two of the industry’s most trusted grains — Red Milo (sorghum) for its smaller kernel size and fast colonization, and Rye Berries for their balanced nutrition and proven reliability.
Culture not included.
Step-by-Step Instructions
Always begin by sanitizing workspace, tools, bags, & gloves.
Step 1: Inoculate (Genetics not included)
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Shake the syringe to evenly distribute the culture before injecting.
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Swab the injection port with 70% isopropyl alcohol; flame-sterilize the needle.
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Inject 5 cc of liquid culture through the self-healing injection port, aiming for the grain layer.
IMPORTANT: If doing multiple bags, briefly re-flame the needle between bags.
Step 2: Incubate
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Let the grain colonize the grain bag for 10–21 days at 70–75°F (timing varies by culture and room conditions).
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At ~30% colonization, gently break up & redistribute the grains to speed even growth.
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Keep the bag in a clean, dark spot with stable room temps.
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Recommendation: Redistribute grains again at ~80% for uniform finish.
For the next stage, you will need: Mycelio Substrate Fruiting Bag.
Always work in a clean environment. If you see or smell contamination, discard the bag safely and do not consume.
These instructions are intended for educational purposes only and apply to the cultivation of lawful gourmet & functional mushroom species only. Misuse of our products for unlawful purposes is strictly prohibited. You are responsible for knowing & complying with your local, state, & federal laws.
Substrate Fruiting Bags
Mycelio's Substrate Fruiting Bags
Fuel your grow with Mycelio’s premium substrate — hydrated to field capacity, pasteurized and sealed. Each bag is fitted with a 0.5-micron filter patch for fruiting in-bag.
Choose from one of our unique blends:
CVA (Coco Coir, Vermiculite, Azomite): Mineral-enriched for balanced moisture and dependable results.
Alient Dirt: Manure-fortified mix that provides extra nutrients to push yields further.
Master’s Mix: Classic hardwood and soy hull blend formulated for consistent colonization and reliable flushes.
Culture not included.
Step-by-Step Instructions
Always begin by sanitizing workspace, tools, bags, & gloves.
Step 1: Transfer & Mix
You have two fruiting options: BagTek or TubTek.
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For BagTek, open the substrate bag, add the colonized grain, and mix thoroughly.
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Seal the bag by folding and taping, using bag clips, or using an impulse sealer.
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For TubTek, mix colonized grain spawn and your substrate together into your monotub.
- Gently pack the substrate down, put the lid on, and remain patient.
Step 2: Secondary Colonization
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Let the mycelium colonize the substrate for 10-14 more days at 70–75°F.
Step 3: Fruit
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Once fully colonized, expose to indirect light and maintain 70–75°F.
Step 4: Harvest
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Using clean scissors, cut away the bag, and harvest when caps are near full expansion.
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Twist gently at the base and pull to remove cleanly.
Always work in a clean environment. If you see or smell contamination, discard the bag safely and do not consume.
These instructions are intended for educational purposes only and apply to the cultivation of lawful gourmet & functional mushroom species only. Misuse of our products for unlawful purposes is strictly prohibited. You are responsible for knowing & complying with your local, state, & federal laws.
All-In-One Grow Bags
Mycelio's All-in-One Mushroom Grow Bags
Take the guesswork out of growing with Mycelio’s All-in-One Mushroom Grow Bags—designed for beginners in mind. Available in 3lb and 5lb sizes, each bag is built for convenience and performance, combining premium ingredients with a state-of-the-art sterile prep process that puts your success first.
Culture not included.
Step-by-Step Instructions
Always begin by sanitizing workspace, tools, bags, & gloves.
Step 1: Inoculate (Genetics not included)
- Shake the syringe to evenly distribute the culture before injecting.
-
Inject 5 cc of liquid culture into the injection port, aiming for the grain layer.
-
We recommend Pioppino, a delicious gourmet mushroom that grows well in our kits!
Step 2: Colonize
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Let the grain colonize for 10–21 days at 70–75°F.
- Break up & redistribute the grains at 30% colonization.
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Keep the bag in a clean, dark spot.
- Recommendation: Redistribute grains again at ~80%.
Step 3: Fruit
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Once fully colonized, cut a slit above the substrate line for additional FAE.
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Keep the bag in indirect light (a shelf or room with natural light is fine).
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Maintain 70–75°F. This part is a waiting game—be patient.
Step 4: Harvest
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Using clean scissors, cut away the bag, and harvest when caps are near full expansion.
-
Twist gently at the base and pull to remove cleanly.
Always work in a clean environment. If you see or smell contamination, discard the bag safely and do not consume.
These instructions are intended for educational purposes only and apply to the cultivation of lawful gourmet & functional mushroom species only. Misuse of our products for unlawful purposes is strictly prohibited. You are responsible for knowing & complying with your local, state, & federal laws.
Two-Stage Grow Kits
Mycelio's Two-Stage Mushroom Grow Kits
Available in Standard or Large sizes, these kits simplify the process without sacrificing results. Mycelio’s beginner-friendly two-stage grow kits include:
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One sterilized milo grain bag
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One fruiting bag with a 0.5-micron filter for optimal gas exchange
Each grain bag pairs with one fruiting bag—just inoculate, mix, and grow.
Culture not included.
Step-by-Step Instructions
Always begin by sanitizing workspace, tools, bags, & gloves.
Step 1: Inoculate (Genetics not included)
- Shake the syringe to evenly distribute the culture before injecting.
-
Inject 5 cc of liquid culture into each grain bag. We recommend Pioppino, a delicious gourmet mushroom that grows well in our kits!
Step 2: Colonize
-
Let the grain colonize for 10–21 days at 70–75°F.
- Break up & redistribute the grains at 30% colonization.
-
Keep the bag in a clean, dark spot.
Step 3: Transfer & Mix
You have two fruiting options: BagTek or TubTek.
-
For BagTek, open the substrate bag, add the colonized grain, and mix thoroughly.
-
Seal the bag by folding and taping, using bag clips, or using an impulse sealer.
-
For TubTek, mix colonized grain spawn and your substrate together into your monotub.
- Gently pack the substrate down, put the lid on, and remain patient.
Step 4: Secondary Colonization
-
Let the mycelium colonize the substrate for 10-14 more days at 70–75°F.
Step 5: Fruit
-
Once fully colonized, expose to indirect light and maintain 70–75°F.
Step 6: Harvest
-
Using clean scissors, cut away the bag, and harvest when caps are near full expansion.
-
Twist gently at the base and pull to remove cleanly.
Always work in a clean environment. If you see or smell contamination, discard the bag safely and do not consume.
These instructions are intended for educational purposes only and apply to the cultivation of lawful gourmet & functional mushroom species only. Misuse of our products for unlawful purposes is strictly prohibited. You are responsible for knowing & complying with your local, state, & federal laws.